Specification for gold potassium cyanide for electroplating 电镀用含金氰化钾规范
Specification for gold i potassium cyanide for electroplating 电镀用氰化亚金钾规范
The positive transformants with the integrates mn - sod gene was identified by zeocin - resistance , pcr screening and expression in p . pastoris . the recombinant mn - sod protein was successfully expressed in pichia pastoris based on the evidences that a relative molecular weight about 23kd appeard in sds - page , the obvious activity of sod existed in native - page and enzymatic activity test , and mn - sod activity was specific base on the inhibition with the mixture of chloroform - enthanol ( 3 : 5 / v : v ) and potassium cyanide . two secreted plasmids ppiczaa - sodm18 and ppiczaa - sodc were constructed and after there linearization were transferred into chromosome of pichia pastoris gs115 by electroporation Pcr鉴定及mut表型分析进一步说明,目标基因已经重组到宿主菌基因组染色体上; 0 . 5甲醇诱导表达后, sds - page结果显示,表达的蛋白相对分子量约为23kd ,活性电泳出现明显活性条带;酶活性测定显示,重组菌株sod活性比对照提高5倍左右;氯仿-乙醇( 3 : 5 v : v )和kcn ( 5mmol l )抑制反应进一步证明,所表达的sod为锰超氧化物歧化酶。
英文解释
a poisonous salt (KCN) used in electroplating and in photography